James Gillespie

• James Gillespie (U.S. politician), U.S. Congressman from North Carolina
• James Gillespie (minister), Scottish minister and theologian
• James Gillespie (footballer), Scottish footballer
• James Gillespie (philanthropist), Scottish snuff and tobacco merchant
• James Gillespie (mariner), HM Excise officer and commander of the cutter Henry Dundas
• James J. Gillespie, member of the Iowa Senate
• James U. Gillespie, member of the Florida House of Representatives